Phosphatase Inhibitor Cocktail-III [100X]

Our Phosphatase Inhibitor Cocktail-III contains phosphatase inhibitors: Sodium fluoride, β-Glycerophosphate, Sodium pyrophosphate, Sodium molybdate, Sodium orthovanadate and other proprietary compound(s) for broad spectrum inhibition of phosphatases that target serine/threonine (Ser/Thr), protein tyrosine, acid and alkaline phosphatases, etc. It can be used in cell and tissue lysates for inhibiting the activity of these phosphatases.

Intact status of the phosphorylated residue of interest is imperative to study the phosphorylation status of specific target proteins. Upon cell lysis for attaining whole cell extracts, a loss of normal cellular signaling regulation occurs, and phosphatases within the cell extract are free to dephosphorylate proteins in an unregulated fashion. The addition of phosphatase inhibitors to the cell lysis buffer aids in the preservation of phosphorylated residues at the time of cell disruption.