Aliases
KPCT; PKC-theta; PKCQ; PRKCQ; PRKCT
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q04759
NCBI Protein: NP_006248.1
Immunogen
Peptide sequence around phosphorylation site of serine 676 (R-L-S(p)-F-A) derived from Human PKCth.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC IF Recommended dilution: Predicted MW: 80kd, Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Background / Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 – 1854. Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253. Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 – 22123. Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 – C119.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser676
