Aliases
KPCT; PKC-theta; PKCQ; PRKCQ; PRKCT
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: Q04759
NCBI Protein: NP_006248.1
Immunogen
Peptide sequence around phosphorylation site of serine 695 (N-F-S(p)-F-M) derived from Human PKCth.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IHC Recommended dilution: Predicted MW: 80kd, Western blotting: 1:500, Immunohistochemistry: 1:50~1:100,
Background / Function
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.Xu ZB, et al.(2004) J Biol Chem 279:50401-50409Thebault S, et al. (2004) Mol Immunol 40: 931-942
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser695
