Aliases
PBP, CRSP1, RB18A, TRIP2, PPARBP
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#: Q15648;
NCBI Gene#: 5469;
N
Immunogen
Peptide sequence around phosphorylation site of Threonine 1457(A-Y-T(p)-P-Q) derived from Human PPAR-BP.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB Recommended dilution: Predicted MW: 168kd, Western blotting: 1:500~1:1000
Background / Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Thr1457
