General Description
Psoralen-(PEO)4-Biotin, a photoreactive reagent used for biotinylation of DNA, RNA and proteins. When activated with ultraviolet (UV) light the psoralen group reacts with thymine and other pyrimidine containing bases to form a covalent bond and does not interfere with hybridization process.
Molecular Formula:Â Â Â C33H44N4 O10S
Molecular Weight:Â Â Â Â Â 688.79
Spacer Arm Length:Â Â Â 36.9 Ã…
Appearance:Â Â Â Â Â Â Â Â Â Â White to off-white solid
Reactive Group:Â Â Â Â Â Â Â Psoralen, reacts upon exposure to UV light
Melting Point:         96-102 °C
Storage Temp:        4-8 °C
Features of Psoralen-(PEO)4-Biotin:
1. Photo-reactive— covalent attachment occurs in the presence of UV light for 10 to 30 minutes
2. Biomolecular labeling— biotinylate DNA or RNA (~ 1 biotin per 10 to 20 bases) without interfering with hybridization
3. Reactive Group — Psoralen-activatedr, eacts through a cyclo-addition mechanism with the 5,6 double bond in thymine and other pyrimidine containing bases
4. Long Spacer Arm 36.9 Å — enables long reach, minimizing steric hindrance for binding interactions with streptavidin
