Description
General Description
Psoralen-(PEO)4-Biotin, a photoreactive reagent used for biotinylation of DNA, RNA and proteins. When activated with ultraviolet (UV) light the psoralen group reacts with thymine and other pyrimidine containing bases to form a covalent bond and does not interfere with hybridization process.
Molecular Formula: C33H44N4 O10S
Molecular Weight: 688.79
Spacer Arm Length: 36.9 Å
Appearance: White to off-white solid
Reactive Group: Psoralen, reacts upon exposure to UV light
Melting Point: 96-102 °C
Storage Temp: 4-8 °C
Features of Psoralen-(PEO)4-Biotin:
1. Photo-reactive— covalent attachment occurs in the presence of UV light for 10 to 30 minutes
2. Biomolecular labeling— biotinylate DNA or RNA (~ 1 biotin per 10 to 20 bases) without interfering with hybridization
3. Reactive Group — Psoralen-activatedr, eacts through a cyclo-addition mechanism with the 5,6 double bond in thymine and other pyrimidine containing bases
4. Long Spacer Arm 36.9 Å — enables long reach, minimizing steric hindrance for binding interactions with streptavidin
Additional information
| Size | 5 mg |
|---|
