Resazurin is a blue non-fluorescent dye that when reduced to the highly red-fluorescent product resorufin (excitation: 530-540 nm; emission: 585-595 nm) can be used as a quantifiable detection agent for enzyme activity assays. The non-fluorescent resazurin can be used as an oxidation-reduction indicator in cell viability assays in a variety of cells, and is applicable in cytotoxicity determination. This dye is routinely utilized for the measurement of metabolic activity and proliferation of living cells. The bioreduction of this dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).
Resazurin sodium salt has been used in viability assay:
• to measure cell viability
• to assess cell proliferation
• to determine viability in high-throughput screenings of microbial natural extracts
