Aliases
Replication factor A protein 2, Replication protein A 34 kDa subunit, REPA2, RPA32, RPA34, RPA2
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
P15927
Immunogen
Recombinant human Replication protein A 32 kDa subunit protein (1-267AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA;Not yet tested in other applications.
Background / Function
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Isotype
IgG
Conjugate
FITC
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Modification
Replication protein A 32 kDa subunit protein
Research
Epigenetics and Nuclear Signaling
Literature
[1] “The primary structure of the 32-kDa subunit of human replication protein A.”Erdile L.F., Wold M.S., Kelly T.J.J. Biol. Chem. 265:3177-3182(1990) [2] “Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201).” Ebert L
