Aliases
DNA-directed RNA polymerase III subunit RPC4; RNA polymerase III subunit C4; DNA-directed RNA polymerase III subunit D; DNA-directed RNA polymerase III 47 kDa polypeptide; RPC53
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P05423
NCBI Gene ID: 661
Immunogen
Synthesized peptide derived from internal of human RPC4.
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
WB Recommended dilution: Western blotting: 1:500~1:3000
Background / Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway By similarity.Ittmann M., Mol. Cell. Biol. 7:3386-3393(1987).Hu P., Mol. Cell. Biol. 22:8044-8055(2002).Kalnine N., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
