Description
Aliases
serum response factor
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot#:P11831;
NCBI Gene#:6722
Immunogen
Peptide sequence around phosphorylation site of serine 77 (L-Y-S(p)-G-S) derived from Human SRF.
Raised In
Rabbit
Species Reactivity
Human Mouse
Tested Applications
WB IHC Recommended dilution: Western blotting: 1:500~1:3000, Immunohistochemistry: 1:50~1:100,
Background / Function
SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5′ of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
Conjugate
Unconjugated
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser77
Additional information
Size | 100μl |
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