Description
Product Description
Streptavidin is a 47KD protein that extracted from streptomyces. Just like avidin, streptavidin has very high affinity to biotin molecule, one million times than the common affinity between antigen and antibody. Avidin is a alkalic protein (IP=10.0-10.5), and it can transfer to be a neutral protein through reconstruction. Isoelectric point of the streptavidin is near to neutrality, IP=6.0~6.5. Thus, streptavidin has very low non-specific absorption to tissue and cell. On the basis of streptavidin, the background of immunohistochemistry analysis is extremely low. With the method of polymeric labeling, streptavidin-HRP can form a complex which composed by about one hundred HRP and fifty streptavidin, and lots of enzymes can ensure the streptavidin-HRP with high sensitivity.
Conjugate: HRP
Form: Concentrated, Liquid
Concentration: 1mg/ml
Storage: At 4˚C for one year.
Application: Dotblot, WB, ELISA
Application details: Dot blot(ECL)|0.25-0.5μg/ml|Western blot(DAB)|0.3-2μg/ml|Western blot(ECL)|0.1-0.3μg/ml|ELISA|0.025-0.05μg/ml|
Additional information
| Size | 0.5ml, 1ml |
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