General description
Sulfo-GMBS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm. These functional groups react with amines and sulfhydryl groups of proteins, respectively, that allow covalent conjugation of amine- and sulfhydryl-containing molecules. Sulfo-GMBS is often used to prepare antibody-enzyme and hapten-carrier protein conjugates in a two-step reaction scheme.
Synonyms:Â Â Â Â Â Â Â Â Â Â Â Â Â Â Sulfo-GMBS sodium salt;Â Sulfo-N-succinimidyl 4-maleimidobutyrate sodium salt
CAS No.:Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 185332-92-7
Molecular Formula:Â Â Â Â Â Â C12H11N2NaO9S
Molecular Weight:Â Â Â Â Â Â Â 328.28
Spacer Arm Length:Â Â Â Â Â Â 7.3 Ã…
Features:
- Reactive groups are Sulfo-NHS ester and maleimide
- Reactive towards amino and sulfhydryl groups
- Non-cleavable
- Water-soluble (compared to GMBS)
- Has a low potential for eliciting crosslinker immune response, ensuring that the primary response to the antigen-carrier protein conjugate is not diluted by a response against a determinant on the crosslinker
- GMBS and Sulfo-GMBS are reported to be less immunogenic than SMCC