Sulfo-NHS-LC-LC-Biotin

General Description

Sulfo-NHS-LC-LC-Biotin is a long amine reactive biotinylation agent with Spacer arm 30.5 Å. It is water-soluble, non-cleavable, and enables simple and efficient biotinylation of antibodies, proteins and primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents.

Molecular Formula:   C₂₆H₄₀N₅O₁₀S₂Na

Molecular Weight:      669.75

Spacer Arm:                30.5 Å

Reactive group(s):      N-hydroxy succinimide ester reacts with primary amines at pH 7.0-9.0

Features of Sulfo-NHS-LC-LC-Biotin:

• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides

• Protein labeling— biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes

• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds

• Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes

• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved