Description
General Description
Sulfo-NHS-LC-LC-Biotin is a long amine reactive biotinylation agent with Spacer arm 30.5 Å. It is water-soluble, non-cleavable, and enables simple and efficient biotinylation of antibodies, proteins and primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents.
Molecular Formula: C26H40N5O10S2Na
Molecular Weight: 669.75
Spacer Arm: 30.5 Å
Reactive group(s): N-hydroxy succinimide ester reacts with primary amines at pH 7.0-9.0
Features of Sulfo-NHS-LC-LC-Biotin:
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
• Protein labeling— biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
Additional information
| Size | 50 mg |
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