Sulfo-NHS-SS-Biotin

General description

Sulfo-NHS-SS-Biotin is a thiol-cleavable amine-reactive biotinylation reagent that contains an extended spacer arm for minimizing the steric hindrances associated with avidin binding. This reagent is water-soluble, thus enabling biotinylation to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents or are complicated by their inclusion. The compound is particularly useful for labeling and purifying cell surface proteins, because its sulfonate group prevents it from permeating cell membranes, and its cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns.