Description
Aliases
MAPT; MTAPT; MTBT1; Neurofibrillary tangle protein; PHF-tau
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P10636
NCBI Protein: NP_001116538.
Immunogen
Peptide sequence around phosphorylation site of serine 356 (I-G-S(p)-L-D) derived from Human Tau.
Raised In
Rabbit
Species Reactivity
Human Mouse Rat
Tested Applications
WB IF Recommended dilution: Predicted MW: 48 62 78 kd, Western blotting: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Background / Function
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.Puig B, et al. (2005) Acta Neuropathol (Berl). 110(3):261-268.DeGiorgis JA, et al. (2005) J Proteome Res. 4(2): 306-315.Alonso Adel C, et al. (2004) J Biol Chem. 279(33): 34873-34881.Kyriakis J M, et al. (1994) Nature. 369: 156-160.
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Modification
Phospho-Ser356
Additional information
| Size | 100?l |
|---|
