Trypsin-EDTA (0.25%), phenol red

Trypsin-EDTA (0.25%), with phenol red, is a cell dissociation solution commonly used to detach adherent cells from culture surfaces in cell culture applications. The 0.25% trypsin concentration effectively breaks down the proteins that bind cells to the culture vessel, while the EDTA chelates calcium and magnesium ions, which are essential for cell adhesion. The phenol red acts as a pH indicator, providing a visual cue for the solution?s pH, making it easy to monitor the dissociation process. This reagent is ideal for routine passaging of adherent cells in research, clinical, and industrial settings.