Aliases
TYH; DYT14; DYT5b; TH;
Antibody Type
Polyclonal Antibody
Uniprot ID
Swiss-Prot: P07101
NCBI Protein: NP_954986.2
Immunogen
Peptide sequence around aa.29~33(V-T-S-P-R)derived from Mouse Tyrosine Hydroxylase.
Raised In
Rabbit
Species Reactivity
Mouse Rat
Tested Applications
WB Recommended dilution: Predicted MW: 55-60kd, Western blotting: 1:500~1:1000,
Background / Function
Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms . This enzyme is required for embryonic development since TH knockout mice die before or at birth . Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH . Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels.Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62. Bodeau-P
Conjugate
Unconjugated
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
