Description
Aliases
Villin-1, VIL1, VIL
Antibody Type
Polyclonal Antibody
Species
Human
Uniprot ID
P09327
Immunogen
Recombinant human Villin-1 protein (1-350AA)
Raised In
Rabbit
Species Reactivity
Human
Tested Applications
ELISA, IHC;Recommended dilution:IHC:1:20-1:200
Background / Function
Epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
Isotype
IgG
Conjugate
Unconjugated
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
Purity
Antigen Affinity Purified
Literature
[1]Helix straightening as an activation mechanism in the gelsolin superfamily of actin regulatory proteins.” Wang H., Chumnarnsilpa S., Loonchanta A., Li Q., Kuan Y.M., Robine S., Larsson M., Mihalek I., Burtnick L.D., Robinson R.C.J. Biol. Chem. 284:21265-21269(2009). [2]Solution structures of the C-terminal headpiece subdomains of human villin and advillin, evaluation of headpiece F-actin-binding requirements.”Vermeulen W., Vanhaesebrouck P., Van Troys M., Verschueren M., Fant F., Goethals M., Ampe C., Martins J.C., Borremans F.A.Protein Sci. 13:1276-1287(2004). [3]Initial characterization of the human central proteome.”Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011).Additional information
| Size | 50μl, 100μl |
|---|
