NHS-Biotin

General Description

NHS-Biotin reacts with primary amines to biotinylate proteins under mild conditions. It has been used to prepare a biotinylated cytidine as a probe in the detection of related DNA sequences, and for biotinylating myeloma cells and immunogens to form a bridge with streptavidin to improve relevant hybridoma formation.

Name:                            N-Hydroxysuccinimido Biotin

Synonyms:                    NHS Biotin; 1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, 2,5-dioxo-1-pyrrolidinyl ester, (+)Biotin N-hydroxysuccinimideester

CAS Number:                35013-72-0

Molecular Weight:       341.38

Color & Form:               White fine-grained powder

Melting Point:               210-212° C

Molecular Formula:     C14H19N3O5S

Spacer Arm:                  13.5 Å

Reactive group(s):        N-hydroxysuccinimide ester reacts with primary amines at pH 7.0-9.0