Description
Product name: Goat α-Rabbit IgG, Alkaline phosphatase (AP) Labeled (1mg/ml)
Host species: Goat
Target species: Rabbit
Conjugation: Alkaline Phosphatase
Tested applications: ICC, IHC-P, ELISA, WB
Form: Liquid
Concentration: 1 mg/ml
Purity: Immunogen affinity purified
Purification notes: This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
Clonality: Polyclonal
Isotype: IgG
Storage buffer: Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
Preservative: 0.1% Sodium Azide
Molar enzyme: Ab protein 1:1
Storage Condition: Shipped at 4°C. Store at +4°C.
Recommended Dilutions: Below are the recommended starting dilutions; optimal dilutions/concentrations and should be determined by the end user:
|
Application |
Recommended Dilution |
|
Western Blot |
1:5000 – 1:50000 Chemiluminescent detection |
|
ELISA |
1:5000 – 1:50000 |
|
IHC-P |
1:100 – 1:1000 |
|
ICC |
Use at an assay dependent dilution |
Western blot – Secondary Antibody: Goat
α-Rabbit IgG Alkaline phosphatase (AP) Labeled
All lanes: Anti-beta Actin antibody –
Loading Control at 1 µg/ml
Lanes 1-6: HeLa (Human epithelial
carcinoma cell line) Whole Cell Lysate, 10 µg protein per lane.
Secondary
Lanes 1 – 2: Goat polyclonal Secondary
Antibody to Rabbit IgG – H&L (AP)
at 1/5000 dilution
Lanes 3 – 4: Goat polyclonal Secondary
Antibody to Rabbit IgG – H&L (AP) at
1/20000 dilution
Lanes 5 – 6: Goat polyclonal Secondary
Antibody to Rabbit IgG – H&L (AP) at
1/50000 dilution
Developed
using the ECL technique
Exposure
time: 3
minutes
Additional information
| Size | 1.0ml |
|---|
