Tris Base

General description

Tris is an organic compound commonly incorporated in multiple molecular biology applications including preparation of buffer solutions such as TAE or TBE for use in electrophoresis protocols. It is highly water- soluble and works well in the pH range 7.0-9.0. It is employed in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers, and can also be used for many running and loading buffers, mainly in combination with glycine and SDS.

Synonyms:                           2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris hydroxymethyl aminomethane, Trometamol

CAS No.:                              77-86-1

Molecular Formula:           NH2C(CH2OH)3

MW:                                      121.14

InChI Key:                            LENZDBCJOHFCAS-UHFFFAOYSA-N

pH range:                             7 – 9

pKa (25 °C):                         8.1

Boiling Point:                       219-220°C/10 mmHg (lit.)

Melting Point:                     167-172°C (lit.)

Absorption:                         ≤0.015 at 300 nm at 100 mg/mL

Storage temp.:                    20-25°C