Campy Blood Free Selective Medium

Campy Blood-Free Selective Medium is a medium used in combination with antibiotics for the detection, isolation and quantification of?Campylobacter jejuni,?C. coli?and?C. lari?in foods.??In this medium, based on the original formulation by Bolton?et?al. (1984), blood is replaced by a mixture of Bacteriological Charcoal, Ferrous Sulfate, Sodium Pyruvate and Casein Peptone. Charcoal detoxifies and reduces oxygen tension. Ferrous Sulfate and Sodium Pyruvate enhance the growth and aerotolerance of?Campylobacter?bacteria. Casein Peptone, together with Gelatin and Beef Extract, provides nutritional compounds such as amino acids, peptides, carbohydrates and vitamins to the bacteria. Sodium Chloride serves as source of essential electrolytes and maintains osmotic equilibrium.??The addition of the selective agents Cefoperazone and Sodium Desoxycholate makes the medium selective for?Campylobacter.