Agarose LE Ultrapure
$185.00 – $1,350.00Price range: $185.00 through $1,350.00
Agarose LE Ultrapure is a highly purified agarose with low EEO, suitable for a variety of molecular biology applications, DNA electrophoresis, etc.
| Size | 100 g, 250 g, 500 g, 1 Kg |
|---|
Agarose is the most popular medium for electrophoresis because of the large size for rapid diffusion and low background. Agarose LE Ultrapure is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analyses of nucleic acids and proteins of various sizes (150 bp to 25 kbp).
Its low EEO (≤ 0.13-mr ) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counter flow) that can result from the presence of excessive sulfate-rich negative ions.
Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols. It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100ml H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.
Formulated for high gel strength and integrity, Agarose LE exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added. Available in 25g, 100g and 500g quantities.
Agarose LE Ultrapure is a multi-purpose agarose with high purity and Low EEO, which is produced in a green, original process without using organic solvents, reducing pollution to the environment and the impact on operators and samples.
Application:
- Molecular Biology Application
- DNA Electrophoresis
Features:
- Appearance: Fine, homogeneous white powder
- Moisture: ≤ 10%
- Gel strength (1% gel): ≥ 1200 g/cm2
- EEO (Electroendosmosis): ≤ 0.13
- Sulfide (SO42-): ≤ 0.15%
- Solubleness: Colorless clear gel liquid
- Ash: ≤ 0.5%
- Solidification temperature (1.5% gel): 36°C±1.5°C
- Thawing temperature (1.5% gel): 88°C±1.5°C
- DNase/RNase: None detected
- Protease: None detected
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