Description
Caspase-4 Assay Kit, Fluorometricwith Ac-LEVD-AFC substrate
General description
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (400 nm); upon cleavage of the substrate by Caspase-4 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
Detection Method: Fluorescence (Ex/Em 400/505 nm)
Species Reactivity: Mammalian
Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis
Storage temp.: -20°C
Features & Benefits:
- Simple one-step procedure that takes only ~1-2 hours
- Fast and convenient
- Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in LEVD-dependent caspase activity
Kit Components :
- Cell Lysis Buffer
- 2X Reaction Buffer
- LEVD-AFC (1 mM)
- DTT (1 M)
Additional information
| Size | 50 Assays, 100 Assays |
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