LDS Sample Buffer [4X]

Description

Our LDS Sample Buffer [4X] contains 564 mM Tris base, 424 mM Tris-HCl, 2.04 mM EDTA, 8 % LDS (Lithium dodecyl sulfate), 40 % Glycerol, 0.88 mM Commassie Brilliant Blue G250, 0.70 mM Phenol red and it doesn’t contain any reducing agent. Coomassie G-250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.

LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris or Tris-Acetate gels.

Use with a protein reducing agent such DTT, TCEP (10 mM; use freshly prepared) or Beta mercaptoethanol (2%) to reduce disulfide bonds in the protein sample.

Additional information