Brilliant Green Agar

Brilliant Green Agar is a selective medium used for the isolation of?Salmonella?spp. other than?S.?typhi. Brilliant Green Agar was first described by Kristensen in 1925 and later modified by Kauffman in 1935. Brilliant Green inhibits Gram-positive and most Gram-negative bacteria, while Phenol Red serves as a pH indicator by changing the color of the medium to yellow when fermentation of Sucrose and Lactose occurs. Proteose Peptone No. 3 and Yeast Extract provide essential nutrients for bacterial growth, while Sodium Chloride supplies electrolytes and maintains osmotic equilibrium. Agar is the gelifying agent.