Anti-RFP Tag, Clonility RF5R

Source protein:           

Protein A affinity
chromatography purified protein from mouse ascites fluid.

Clonality and Host Species:    

RF5R, Mouse

Immunogen and Isotype:        

RFP from the Discosoma
sp. (FLAG) synthetic N-terminal
peptide conjugated to KLH, IgG1

Specificity and reactivity:       

Detects the native and
denatured forms of RFP and its variants such as TagRFP, Turbo RFP ds RED
mCherry, mOrange .

Application:                              

Western blot, Dot blot,
ELISA, IHC/IS, Western blot (with ECL) 1-10 ng of RFP or RFP fusion protein.


Available form:                        

Lyophilized 1mg/mL (lyophilized
in 10mM PBS, pH 7.2 with 1% BSA and 0.05% Sodium azide; NaN₃)

                                       
Working procedure:                

Western blot (with ECL)
1:1000-3,000 dilution incubate for 1 hour at room temperature. For the best
results, determine the optimal working antibody dilution for your protein of
interest.

Storage:                                

Supplied lyophilized
antibodies can be stored at 20⁰C for 3 years.

Reconstitute the
lyophilized antibody with distilled water to 1mg/ml or a desired concentration
before use. 

Reconstituted liquid can be
stored at -20^oC for 2 years. Centrifuge after first thaw to maximum
product recovery. Aliquot into smaller volume to avoid repeated freeze-thaw
cycles.  Store aliquots at 4⁰C
for several days to 2 weeks.