Protein Marker – Tricolor (10-245 kDa)

General Description

Our Tricolor Protein Marker is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights, for 10 to 245 kDa.

Proteins are covalently coupled with a blue chromophore, except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Tri-color Prestained Protein Ladder is designed for monitoring proteins separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.

It is a mixture of nine blue-, red-, and green-stained proteins (10 to 245 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.

Applications

• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots