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DMEM/F-12 GlutaPlus No HEPES

Price range: $46.00 through $324.00

Size500 mL, 2×500 mL, 4×500 mL, 6×500 mL, 10×500 mL
SKU: G4610 Categories: ,
Description

Product Description

DMEM/F-12 medium was modified by mixing DMEM with Ham’s F-12 medium at a ratio of1:1. The modified DMEM/F-12 medium is richer in nutrients and contains more micronutrients, which is a widely used basal medium for supporting the growth of many different mammalian cells, as well as for the culture of mammalian cells at low serum levels and for clonal density culture. DMEM/F-12 has also been used as a base medium for the development of serum-free medium.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Add 5-10% serum or serum-free additives depending on the cell type.

This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 17.5 mM D-glucose,2.5 mML-Alanyl-L-Glutamine,0.5 mM sodium pyruvate, phenol red indicator, no HEPES.


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves and protective clothing.

For Research Use Only!

G4610DMEM/F-12Formula

ComponentsConcentrationComponentsConcentration
mg/LmMmg/LmM
Amino acidsInorganic salts
Glycine18.750.250Calcium Chloride (CaCl2)(anhyd.)116.61.050
L-Alanine4.450.050Cupric Sulfate (CuSO4·5H2O)0.00130.0000052
L-Arginine monohydrochloride147.50.699Ferric Nitrate (Fe(NO3)3·9H2O)0.050.0001238
L-Asparagine monohydrate7.50.050Ferrous Sulfate (FeSO4·7H2O)0.4170.0015
L-Asparagine6.650.050Magnesium chloride (MgCl2)(anhyd.)28.640.301
L-Cysteine hydrochloride hydrate17.560.100Magnesium sulfate (MgSO4) (anhyd.)48.840.407
L-Cystine dihydrochloride31.290.100Potassium Chloride (KCl)311.84.157
L-Glutamic acid7.350.050Sodium bicarbonate(NaHCO3)243829.02
L-Alanyl-L-Glutamine543.02.500Sodium chloride (NaCl)6995.5120.6
L-Histidine hydrochloride monohydrate31.480.150Disodium (hydrogen) phosphate(Na2HPO4)(anhyd.)71.020.500
L-Isoleucine54.470.416Sodium dihydrogen phosphate(NaH2PO4)(anhyd.)54.340.453
L-Leucine59.050.451Zinc Sulfate(ZnSO4·7H2O)0.4320.0015
L-Lysine hydrochloride91.250.499Other components
L-Methionine17.240.116D-Glucose (Dextrose)315117.51
L-Phenylalanine35.480.215Hypoxanthine Na2.390.015
L-Proline17.250.150Linoleic Acid0.0420.00015
L-Serine26.250.250Lipoic acid0.1050.00051
L-Threonine53.450.449Phenol Red8.10.022
L-Tryptophan9.020.044Putrescine dihydrochloride0.0810.00050
L-Tyrosine disodium salt55.790.214Thymidine0.3650.00151
L-Valine52.850.452Sodium pyruvate550.500
VitaminsHEPES––
Biotin0.00350.0000143
Choline chloride8.980.06414
D-Calcium pantothenate2.240.00470
Folic Acid2.650.00601
Niacinamide2.020.01656
Pyridoxine hydrochloride2.0130.00977
Riboflavin0.2190.00058
Thiamine hydrochloride2.170.00644
Vitamin B120.680.00050
i-Inositol12.60.07
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Certificate of Analysis
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Safety Datasheet

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Certificate of Analysis

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