Nickel-IDA Agarose Resin

General Description

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates. Nickel-IDA Agarose Resin contains IDA (iminodiacetate, a tridentate chelating agent), covalently coupled to 6% highly cross-linked agarose beads ligated by stable ether linkages via a spacer arm. This resin utilizes nickel (Ni2 ) for the purification of 6X histidine tagged proteins and binds to six histidine residues (6X His), a common tag used in protein purification.

Our Ni-IDA Agarose Resin is ideal for rapid purifications of His-tagged proteins as it is loaded with Ni2+ and the recognizes two exposed histidines for an appreciable retention of a protein with a 4X or 6X His tag. The protein purity and yield is dependent on working conditions, size, conformation, expression levels, solubility and sample impurities.

Features:

• Excellent binding capacity based on high surface area to volume ratio
• Fast flow rates
• Notable mechanical and chemical stability
• Reusable without any significant decrease in yield