VPS4A Antibody

Aliases Vacuolar protein sorting-associated protein 4A, Protein SKD2, VPS4-1, hVPS4, VPS4A Antibody Type Polyclonal Species Human Uniprot ID Q9UN37 Immunogen Recombinant human Vacuolar protein sorting-associated protein 4A protein (1-120AA) Raised In Rabbit Species Reactivity Human,mouse,rat Tested Applications ELISA, WB, IHC;Not yet tested in other applications. Background / Function Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). Isotype IgG Conjugate Unconjugated Storage Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Form Liquid Storage Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. Purity Antigen Affinity Purified Modification Vacuolar protein sorting-associated protein 4A