Trypsin-EDTA (0.5%), phenol red

Trypsin-EDTA (0.5%), with phenol red, is a potent cell dissociation solution designed for the efficient detachment of adherent cells from culture surfaces. The 0.5% trypsin concentration provides stronger enzymatic activity to break down the proteins responsible for cell adhesion, while the EDTA chelates calcium and magnesium ions, further facilitating the dissociation process. Phenol red serves as a pH indicator, allowing researchers to easily monitor the solution?s pH and adjust the timing of cell detachment. This formulation is ideal for cell lines that require a stronger dissociation solution for subculture, passaging, or other experimental procedures.