Phloroglucinol Stain
Price range: $28.00 through $175.00
Phloroglucinol Stain or Wiesner Reagent is intended for the qualitative detection and visualization of lignified structures in plant tissue sections.
| Size | 125 mL, 250 mL, 500 mL, 1 L |
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Phloroglucinol Stain (Wiesner Reagent) is intended for the qualitative detection and visualization of lignified structures in plant tissue sections. The Wiesner reaction is widely used in plant anatomy, botanical research, and histochemical studies to identify lignin-associated structures, including xylem vessels, fibers, and other lignified cell walls.
Phloroglucinol Stain is used to detect lignin in plant tissues. It is especially valuable for plant cell wall studies, as it stains lignin-rich regions of the tissue, aiding in research related to plant biology, including the study of cell wall composition and structure.
The kit uses phloroglucinol dissolved in ethanol and hydrochloric acid supplied separately. This two-component format avoids prolonged storage of the combined acidic staining reagent and allows the reagents to be combined during the staining procedure.
In the presence of hydrochloric acid, phloroglucinol reacts with certain lignin-associated cinnamaldehyde structures, producing a characteristic red coloration. The resulting staining pattern assists researchers in examining lignification and plant tissue organization.
Key Features and Benefits:
- Lignin visualization: Produces a characteristic pink-to-red staining reaction in responsive lignified plant tissues.
- Research applications: Suitable for plant anatomy, histochemistry, and lignification studies.
- Convenient preparation: Phloroglucinol is supplied as a prepared alcoholic solution.
- Flexible laboratory use: Separate reagents allow preparation of the acidic staining system at the time of use.
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