Glycoprotein Staining Kit

Our Glycoprotein Staining Kit detects glycoprotein sugar moieties in polyacrylamide gels and on nitrocellulose membranes with the use of enhanced Periodic acid-Schiff method. The glycols present in the glycoproteins are oxidized to aldehydes when treated with an oxidizing agent; Periodic acid. The aldehyde group reacts with Glycoprotein Stain, yielding magenta color bands with a light pink or colorless background.

Glycoproteins consist of carbohydrate moieties covalently linked to a polypeptide backbone and serve a variety of functions. They are found in cell membranes, connective tissues, blood, and secretions. These diverse proteins may have a carbohydrate component that represents <1% to >80% of the total weight. Sugars that commonly occur in glycoproteins include galactose, mannose, glucose, N-acetylglucosamine, N-acetylgalactosamine, sialic acid, fucose and xylose. This stain selectively binds to glycosylated proteins, making it an important tool for research on cell signaling, protein glycosylation, and diseases associated with glycoprotein abnormalities.

Cat. No. 66714-0 is Suitable for 10 mini gels of size 8 x 8cm or 20 Nitrocellulose membrane of size 8 x 8cm and Cat. No. 66714-1 for double quantity.

Features:

• Standardized protocol to achieve optimal staining in less than 2 hours

• Glycoprotein specific staining – detects glycosylated proteins with sialic acid and other oxidizable carbohydrate groups

• Specificity of the Color – glycoproteins are detected as magenta bands against a colorless or faint pink background

• Supplied with Controls – a Positive control (Horseradish peroxidase) and Negative control (Soybean Trypsin Inhibitor) proteins are included in the kit

SAFETY Note for User:

The kit components Glycoprotein Stain and Oxidizing Reagent are highly CORROSSIVE in nature, handle them very carefully using protective gear and refer to Safety Data Sheet before using the kit.