Aniline Blue 2.5% In 2% Acetic Acid

Aniline Blue 2.5% in 2% Acetic Acid is a specialized stain used primarily for highlighting collagen fibers and extracellular matrix components in tissue samples. This solution is commonly utilized in histological procedures such as the Masson’s Trichrome stain, which is employed to differentiate collagen from muscle and cytoplasm. The aniline blue provides a strong blue coloration to collagen, enabling clear contrast and visualization under a microscope. The addition of acetic acid helps stabilize the dye and maintains the tissue’s pH, ensuring reliable and consistent results.